). Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. 5. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. MOLECULAR DEVICE M5. Fig. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Statistical analysis. 312 gain calibration failed. 9000-0756. 10× trypsin 0. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. Exhibit with us {{>currencyLabel}} Back. This assay measures the two major sugars in wine, glucose and fructose. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. 314 low lamp level warning. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. , Sunnyvale, CA, USA) at 595 nm. 0 to 4. If depot service is the best option for you we will assist you with logistics to help you keep. , Sunnyvale, CA). density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). TDP-43 stress granular cells were treated with 70% ice-cold. 3. 190–850 nm <± 1. 4. Each experiment was performed in duplicate. 1. Summary of Contents for Molecular Devices SPECTRAmax 340PC. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. MCE has not independently confirmed the accuracy of these methods. Upgradeable modules are also available including western blot, cell imaging, and fast. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 340/70 nm. Stat Fax 3200 Microplate Reader. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. Alkaline phosphatase (ALP) activity. SpectraMax Multi-Mode Microplate Readers. 0200-0729. Wavelength Range. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. Number of flashes. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Microplate Spectrophotometer Operator’s Manual. The. A 0. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 1% N-1-naphtylethylenediamine dihydrochloride, 2. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. Warranty. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. Rate of fibrinolysis. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Stat Fax 3200 Microplate Reader. Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. Incubation with TMB substrate was done for 18 min. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. The VersaMax and SpectraMax 190 read 96-well microplates. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. SpectraMax 384 Plus. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Fibrin clots were formed as described above with some modifications 46. Data were expressed as mean percentage of viable cells. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. 3 mm. Amino Acid Analysis. 1 mL of acetylacetone. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. Bioz Stars score: 86/100, based on 1 PubMed citations. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Unit powers. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. 0200-7027. Calculating % of total mediator release in RBL assay. SpectraMax Multi-Mode Microplate Readers. chen@case. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. 5. 2 - 3. 5 stars - 1602 reviewsThis assay typically develops in 1. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Or scan up to 384 wells at any time to determine the best assay conditions. Homeostatic model assessment for insulin. 2 were considered negative for IgM antibody. Revvity. Molecular Devices Spectramax 340PC instructions. , Sunnyvale, CA, USA) at 595 nm. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. SpectraMax Microplate Readers. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. #N207) LAL Reagent Water (Lonza cat. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Microplate Readers. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). The SPECTRAmax 340PC can measure. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Also for: Spectramax 190, Spectramax 340pc384, Versamax. Driessen*,MaaikeA. Donor: 616/10 nm. 315 can't find zero order. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). 5. D2. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. 2. Software (SoftMax Pro ® software or comparable plate reader software). 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. (Shiga, Japan) and kept for 30 min in the dark. Assay buffer was 50 m M Hepes, pH 7. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. These lab workhorses are used in drug discovery, research, bioassay validation, and. ScanLater Western Blot Detection Cartridge. reader (Molecular Devi ces). 1 mL of culture media with 10% FBS. SpectraMax QuickDrop Micro-Volume Spectrophotometer. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. The ABS reader ranges from. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. Awareness Technology Inc. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. , Sunnyvale, Calif. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. ). The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. Microplate Reader Shelf. \r plates can be used in the SPECTRAmax PLUS 384. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. 4. 2. 340 nm / 80 nm. 003 OD Stray light ≤ 0. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. Read one sample or up to 384. RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. 5 ng/ml; estradiol: 10 pg/ml. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 0 OD. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. 00. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. 94 g calcium chloride dihydrate (both. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). 0. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. , Fremont, CA, USA). 2. Dissolve 13. Both readers feature an 8-channel read head for fast reads and. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). Fully tested with Warranty. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Donor: 620 nm. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. The growth. 5% phosphoric acid) in microplates at room temperature for 10 min. 7 macrophages were obtained from ATCC (Manassas, VA). Microplate Spectrophotometer Operator’s Manual. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. S. Buffer (100 mmol L –1acetate buffer, pH 5. Unit has several areas of damaged plastic. 4; 100 m M NaCl, 0. 04. Emission. Spectrophotometer (SpectraMax 340). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. HTRF-compatible SpectraMax Multi-Mode Readers. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. plates can be used in the SPECTRAmax PLUS 384. 05 ng/mL; progesterone: 2. The first time you replace an OEM lamp you must use the N-34-MLA. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . used. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. control or celatrol-treated cells. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Streamline GLP/GMP compliance. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). Awareness Technology, Inc. Tunable for filterless flexibility. Heme was preincubated for 5 min with A when added before the metals. Versteegh, Yoran H. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. , Sunnyvale, CA). Details. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. Stat Fax 4700 Microstrip Reader. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. SpectraMax Injector Cartridge with SmartInject™ Technology. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. 00 USD. 304 signal level saturation. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Call 1-800-745-2710. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). Wavelength Selection. reader (SpectraMax 340; Molecular devices, LLc). For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. Endpoint, kinetic, spectrum. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Anti-proteolytic activity. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). California Lightworks uses only the highest quality LEDs from Osram. Quick View. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). One plate carrier adapter is provided with the instrument. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). gp70 blocking. Briefly, cells were seeded into a. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). To measure the cell biomass (g/L),. 30 µs. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. . Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 4. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. SpectraMax Microplate Readers. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). 2. 2. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). Softmax PRO v5. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). Assay setup for a 384-well low-volume plate. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). The assays contained 0. Service parts for these models are not being produced and are no longer in stock. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. Spectral Range. Alves, Amelia K. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Each SpectraMax® 340 PC 384 Microplate Reader has an. TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. AoSMCs were seeded into 6-well culture plates. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. SpectraMax ® Plus 384 optics. The spectrophotometers used were SpectraMax 340 (for GSH, GCS, and HNE-His assays) and SpectraMax M3 (for GGT, GPx, and GST assays), both from Molecular Devices, Sunnyvale, CA. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). or microplate reader (e. The area under the curve (AUC) for the comparison of the growth rates was determined using. View the Molecular Devices website. Quick View. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. TDP-43 stress granular cells were treated with 70% ice-cold. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. 2. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Features showcased by this model include. Operators can conveniently select up to 6 wavelengths at a time for. Intra- and inter-assay coefficients of variation were lower than 8.